Dictionary of Allergies .. ADVIA Centaur specific IgE assay

The ADVIA Centaur specific IgE assay is a reverse sandwich immunoassay (Fig. 1) using direct chemiluminescent technology. The monoclonal mouse antihuman IgE antibody is covalently bound to paramagnetic particles (PMP) in a solid phase. The anti-IgE coupled to the PMP captures the sample IgE. Non-IgE is removed by washing. Biotinylated allergen is added in excess and binds to the allergen-specific IgE antibody captured on the solid phase. Acridimium ester-labeled streptavidin in the LITE reagent binds to the biotin-labeled allergen. A direct relationship exists between the amount of allergen-specific IgE present in the sample and the amount of relative light units detected by the system.[1]. The ADVIA Centaur sIgE assay can be a convenient and efficient method to be used in medium- to large-sized laboratories[2].

References

1. Ricci, G., Capelli, M., Miniero, R., Menna, G., Zannarini, L., Dillon, P. & Masi, M. A comparison of different allergometric tests, skin prick test, Pharmacia UniCAP® and ADVIA Centaur®, for diagnosis of allergic diseases in children.,Allergy 2003:58 (1), 38-45.
2. Kim HS, Choi SK, Ko YS, Chung D, Kim JM, Kim YJ, Choi J, Kim D, Suh JW, Ahn YH. Evaluation of Allergen Specific IgE assay on ADVIA Centaur Immunoassay System. Korean J Lab Med. 2006 Oct;26(5):362-8